Fluorescence Microscopy

Fluorescence Microscopy utilizes light of specific wavelength to excite (excitation) fluorophores bound to biological samples, to emit (emission) light at longer wavelength for analysis. Materion Balzers Optics fluorescence filter sets enable this interaction and include excitation filters, emission filters (bandpass, shortpass or longpass) and dichroic beamsplitters. Cut-on and cut-off wavelengths of the filters are optimized for the excitation wavelength and the fluorescent dye. The filter performance is realized by all-dielectric hard coatings deposited onto a single substrate. Since absorbing glasses and filter mounts are not needed, there is high flexibility for the filter dimensions in compact optical set-ups.